Review



pgex 2t vector  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Addgene inc pgex 2t vector
    Pgex 2t Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+2t+vector/pGEX-RhoA+(Plasmid+%2312202)/pm40334503-302-7-14
    Average 92 stars, based on 6 article reviews
    pgex 2t vector - by Bioz Stars, 2026-09
    92/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: DLC1 SAM domain-binding peptides inhibit cancer cell growth and migration by inactivating RhoA
    Article Snippet: .. Expression constructs DNA sequence encoding the DLC1-SAM (residues 13–78) was subcloned into the pGEX-2T vector (Addgene) for the expression of GST-SAM in Escherichia coli . ..

    Article Title: Protocol for structural and biochemical analyses of RhoA GTPase
    Article Snippet: .. Obtain the expression plasmid encoding human full length RhoA in pGEX-2T vector from Jonathan Chernoff (Addgene Plasmid # 12202, https://www.addgene.org ) ( ) and sequence with pGST 5′ and 3′ primers to confirm. ..

    Construct:

    Article Title: DLC1 SAM domain-binding peptides inhibit cancer cell growth and migration by inactivating RhoA
    Article Snippet: .. Expression constructs DNA sequence encoding the DLC1-SAM (residues 13–78) was subcloned into the pGEX-2T vector (Addgene) for the expression of GST-SAM in Escherichia coli . ..

    Sequencing:

    Article Title: DLC1 SAM domain-binding peptides inhibit cancer cell growth and migration by inactivating RhoA
    Article Snippet: .. Expression constructs DNA sequence encoding the DLC1-SAM (residues 13–78) was subcloned into the pGEX-2T vector (Addgene) for the expression of GST-SAM in Escherichia coli . ..

    Article Title: Protocol for structural and biochemical analyses of RhoA GTPase
    Article Snippet: .. Obtain the expression plasmid encoding human full length RhoA in pGEX-2T vector from Jonathan Chernoff (Addgene Plasmid # 12202, https://www.addgene.org ) ( ) and sequence with pGST 5′ and 3′ primers to confirm. ..

    Plasmid Preparation:

    Article Title: DLC1 SAM domain-binding peptides inhibit cancer cell growth and migration by inactivating RhoA
    Article Snippet: .. Expression constructs DNA sequence encoding the DLC1-SAM (residues 13–78) was subcloned into the pGEX-2T vector (Addgene) for the expression of GST-SAM in Escherichia coli . ..

    Article Title: Protocol for structural and biochemical analyses of RhoA GTPase
    Article Snippet: .. Obtain the expression plasmid encoding human full length RhoA in pGEX-2T vector from Jonathan Chernoff (Addgene Plasmid # 12202, https://www.addgene.org ) ( ) and sequence with pGST 5′ and 3′ primers to confirm. ..

    Article Title: Identification of a new small Rho GTPase inhibitor effective in glioblastoma human cells.
    Article Snippet: Glioblastoma (GBM) is the most common and lethal primary brain tumour.. The prognosis for GBM patients remains poor due to rapid tumour recurrence and resistance to conventional treatments.. Small Rho GTPase proteins, which regulate cell shape and motility, are critical for GBM aggressive growth and infiltration into the surrounding brain parenchyma.

    Clone Assay:

    Article Title: Identification of a new small Rho GTPase inhibitor effective in glioblastoma human cells.
    Article Snippet: Glioblastoma (GBM) is the most common and lethal primary brain tumour.. The prognosis for GBM patients remains poor due to rapid tumour recurrence and resistance to conventional treatments.. Small Rho GTPase proteins, which regulate cell shape and motility, are critical for GBM aggressive growth and infiltration into the surrounding brain parenchyma.

    Recombinant:

    Article Title: Discovery of novel chemical scaffolds as RhoA inhibitors using virtual screening, synthesis, and bioactivity evaluation
    Article Snippet: RhoA has been implicated in diverse cellular functions and is a potential cancer therapeutic target.. Through virtual screening, we have identified a RhoA inhibitor, DDO-5701.. DDO-5701 has an affinity to RhoA at the micromolar level in vitro.

    Generated:

    Article Title: RSK2 drives cell motility by serine phosphorylation of LARG and activation of Rho GTPases
    Article Snippet: .. A GST-fused RhoA-binding domain (RBD) of Rhotekin protein in pGEX-2T vector (pGEX-2T-RBD) was purchased from Addgene as probe for the pulldown of GTP-RhoA, while a GST-RhoA-G17A in pGEX-4T1 vector was generated by mutagenesis as probe to retrieve activated LARG ( ). .. The recombinant GST-fused proteins were expressed in Escherichia coli strain BL21-DE3 (lysE) as described in the “recombinant protein expression section,” and then extracted and coupled to glutathione-agarose (Sigma) resin following the protocol described for the preparation of GST-Raf-RBD–coupled glutathione resin ( ).

    Mutagenesis:

    Article Title: RSK2 drives cell motility by serine phosphorylation of LARG and activation of Rho GTPases
    Article Snippet: .. A GST-fused RhoA-binding domain (RBD) of Rhotekin protein in pGEX-2T vector (pGEX-2T-RBD) was purchased from Addgene as probe for the pulldown of GTP-RhoA, while a GST-RhoA-G17A in pGEX-4T1 vector was generated by mutagenesis as probe to retrieve activated LARG ( ). .. The recombinant GST-fused proteins were expressed in Escherichia coli strain BL21-DE3 (lysE) as described in the “recombinant protein expression section,” and then extracted and coupled to glutathione-agarose (Sigma) resin following the protocol described for the preparation of GST-Raf-RBD–coupled glutathione resin ( ).

    Countercurrent Chromatography:

    Article Title: Inhibition of E-cadherin/catenin complex formation by O-linked N-acetylglucosamine transferase is partially independent of its catalytic activity.
    Article Snippet: .. The fragment between BamHI and EcoRI in the pGEX-2T vector (Addgene; Cambridge, MA, USA) containing the GST tag was replaced by a linker (5'-GGA TCC GGT GGT ACC GCT AGC GCG GCC GCT CCC GGG GAA TTC-3') containing restriction sites for BamHI, KpnI, NheI, NotI, SmaI and EcoRI, followed by digestion with KpnI and SmaI. ..

    other:

    Article Title: DLC1 SAM domain-binding peptides inhibit cancer cell growth and migration by inactivating RhoA
    Article Snippet: Soft agar wells were stained for 1 h with crystal violet (Sigma-Aldrich) and washed in PBS.



    Similar Products

    93
    Cytiva Europe pgex 2t vector
    Pgex 2t Vector, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+2t+vector/pGEX+Vectors+PGEX-2T/us12378567-254-18-20
    Average 93 stars, based on 1 article reviews
    pgex 2t vector - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    86
    Thermo Fisher pgex 2t expression vector
    Pgex 2t Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+2t+vector/pm38139191-226-22-25
    Average 86 stars, based on 1 article reviews
    pgex 2t expression vector - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    92
    Addgene inc pgex 2t vector
    Pgex 2t Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+2t+vector/pGEX-RhoA+(Plasmid+%2312202)/pm40334503-302-7-14
    Average 92 stars, based on 1 article reviews
    pgex 2t vector - by Bioz Stars, 2026-09
    92/100 stars
      Buy from Supplier

    93
    Cytiva Europe pgex 2t
    Pgex 2t, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+2t+vector/pGEX+Vectors+PGEX-2T/10__7554_slash_elife__90309-520-46-48
    Average 93 stars, based on 1 article reviews
    pgex 2t - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    Cytiva Europe plasmid pgex2t gst
    Plasmid Pgex2t Gst, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+2t+vector/pGEX+Vectors+PGEX-2T/pmc12001331-1057-0-3
    Average 93 stars, based on 1 article reviews
    plasmid pgex2t gst - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    90
    Merck & Co pgex-2t vector
    Pgex 2t Vector, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+2t+vector/ge28/pm39834079-269-14-17
    Average 90 stars, based on 1 article reviews
    pgex-2t vector - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    86
    Cytiva Europe vector pgex 2t
    Vector Pgex 2t, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+2t+vector/bio_rxiv__2024__07__09__602786-270-10-12
    Average 86 stars, based on 1 article reviews
    vector pgex 2t - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    90
    Addgene inc pgex-2t vectors
    Pgex 2t Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+2t+vector/pgex+2t++ha+hpv16e7/pm38494697-82-11-13
    Average 90 stars, based on 1 article reviews
    pgex-2t vectors - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    86
    Danaher Inc pgex 2t vector
    Pgex 2t Vector, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+2t+vector/pmc10862391-154-8-10
    Average 86 stars, based on 1 article reviews
    pgex 2t vector - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    90
    Thermo Fisher pgex-2t expression vector
    Pgex 2t Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+2t+vector/pmc10743750-166-22-25
    Average 90 stars, based on 1 article reviews
    pgex-2t expression vector - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results